Time resolved measurements show that phosphate release is the rate limiting step on myofibrillar ATPases
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چکیده
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Phosphate release contributes to the rate-limiting step for unwinding by an RNA helicase
RNA helicases function in numerous aspects of RNA biology. These enzymes are RNA-stimulated ATPases that translocate on RNA and unwind or remodel structured RNA in an ATP-dependent fashion. How ATP and the ATPase cycle fuel the work performed by helicases is not completely clear. The hepatitis C virus RNA helicase, NS3, is an important model system for this class of enzymes. NS3 binding to a si...
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Under conditions where cytochalasin B induces ATPase activity of monomeric actin (0.3 mM MgCl2, 1 mM EGTA, 30 microns cytochalasin B, 1 mM ATP) the rate constant of the exchange of actin-bound epsilon-ATP for free ATP is about 4-6 times faster than steady state ATPase activity. When a stoichiometric ATP-actin complex is extracted with PCA (single turnover experiment) the apparent rate constant ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1995
ISSN: 0014-5793
DOI: 10.1016/0014-5793(95)00356-e